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Prasad, A. N. and Seshia, S. S. Status epilepticus in pediatric practice: neonate to adolescent. Adv.Neurol. 2006;97:229-243. View abstract.
An inherited condition in which the body cannot process biotin (biotinidase deficiency): People with this condition might have low levels of biotin and may need a biotin supplement.
In an attempt to reduce the problems associated with endogenous biotin in conventional tyramide signal amplification, a biotin-free system, fluorescyl-tyramide amplification system (FT-CSA or CSAII), was introduced. Rather than biotinyl-tyramide, this system uses fluorescyl-tyramide and does not contain avidin/biotin reagents avoiding the problem associated with endogenous biotin. In this method, addition of primary antibody is followed by a peroxidase-labeled secondary antibody. Peroxidase enzyme is responsible to catalyze the transformation and deposition of fluorescyl-tyramide in the tissue section. When the reaction terminates, it could be inspected by fluorescence microscopy. The produced signals could even be converted to a colorimetric reaction by using peroxidase-conjugated antifluorescein antibody and a diaminobenzidine-hydrogen peroxide substrate. Branger P, Parienti JJ, Derache N, Kassis N, Assouad R, Maillart E, Defer G. Relapses During High-Dose Biotin Treatment in Progressive Multiple Sclerosis: a Case-Crossover and Propensity Score-Adjusted Prospective Cohort. Neurotherapeutics. 2020. View abstract. Tsao, C. Y. Current trends in the treatment of infantile spasms. Neuropsychiatr.Dis.Treat. 2009;5:289-299. View abstract.
Albarracin C, Fuqua B, Evans JL, Goldfine ID. Chromium picolinate and biotin combination improves glucose metabolism in treated, uncontrolled overweight to obese patients with type 2 diabetes. Diabetes Metab Res Rev 2008;24:41-51. View abstract. Pacheco-Alvarez D, Solórzano-Vargas RS, Del Río AL. Biotin in Metabolism and Its Relationship to Human Disease. Arch Med Res 2002;33(5):439-47. View abstract. Brenner, S. and Horwitz, C. Possible nutrient mediators in psoriasis and seborrheic dermatitis. II. Nutrient mediators: essential fatty acids; vitamins A, E and D; vitamins B1, B2, B6, niacin and biotin; vitamin C selenium; zinc; iron. World Rev.Nutr.Diet. 1988;55:165-182. View abstract. Demas A, Cochin JP, Hardy C, Vaschalde Y, Bourre B, Labauge P. Tardive Reactivation of Progressive Multiple Sclerosis During Treatment with Biotin. Neurol Ther. 2019;9(1):181-185. View abstract.Biotin is a water-soluble vitamin of the vitamin B family, also known as vitamin B7 or H. Your body needs it to process nutrients into energy and maintain a healthy nervous system. It also plays an important role in healthy hair, skin, and nails. Hair loss, brittle nails, and rashes are signs of biotin deficiency, which can occur due to genetics, poor diet, alcoholism, or pregnancy. Cree BAC, Cutter G, Wolinsky JS, et al. Safety and efficacy of MD1003 (High-dose biotin) in patients with progressive multiple sclerosis (Spi2): a randomised, double-blind, placebo-controlled, phase 3 trial. Lancet Neurol. 2020;19(12):988-997. doi: 10.1016/S1474-4422(20)30347-1. If you have a biotin deficiency, for instance, some women can be slightly deficient in biotin when they are pregnant or breastfeeding, it can take several months for you to start seeing a difference with your hair. The improvements are likely to be subtle too. 10 Can biotin cause hair loss?
As a further general rule, one should always try to use the simplest detection method with sensitivity enough for detection of the antigen. The multilayering of detection antibodies beyond this threshold can be problematic as with the addition of every new step, the risk of nonspecific interaction with the preparation increases. There are some exceptions to this rule. For example, in tumor-specific antigens, which are not expressed in normal condition, the use of more sensitive methods might decrease detection level cutoff and increase the likelihood for early detection of cancer [ 143, 144]. Gao Y, et al. (2016). New perspective on impact of folic acid supplementation during pregnancy onneurodevelopment/autism in the offspring children — A systematic review. Cleavable biotinylation reagents:Certain biotinylation reagents contain a disulfide bond in their spacer arms or conjugate to protein via disulfide bonds. Biotinylated proteins can be eluted from the affinity resin by cleaving the disulfide bond with reducing agent. The biotin group is left bound to the resin, and the protein is recovered (free of any biotin label). The Cell Surface Protein Isolation Kit uses this strategy (labeling with Sulfo-NHS-SS-Biotin).Most people can take biotin supplements without experiencing any side effects. However, it is possible for people to take them and experience 13: Nuts and seeds have biotin, but how much you get depends. For instance, a one-fourth cup of roasted almonds has 1.5 micrograms, but just six walnut halves have 9.5 micrograms. A one-fourth cup of roasted sunflower seeds has 2.6 micrograms of biotin, more than the amount present in any other seed. But in a few cases when people have taken large amounts of vitamin B6, particularly for more than a few months, the effect can be permanent. Rasoulzadeh Z et al. Placental kisspeptins differentially modulate vital parameters of estrogen receptor-positive and-negative breast cancer cells. PLoS One. 2016; 11(4):e0153684
Vitamin B complex appears to be safe for most people to take as the body does not store any excess but eliminates it in urine. When taking supplements, however, it is essential to follow the instructions as taking too much of some B vitamins may have adverse effects. Is vitamin B complex the same as B12?
Avidin-Biotin Technical Handbook
Pantothenic acid cannot be stored in the body, so you need it in your diet every day. What happens if I take too much pantothenic acid?
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